Recombinant humaan transgeline 2/TAGLN2-eiwit

TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Recombinant human TAGLN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Transgelin-2, HA1756, KIAA0120, Transgelin 2, SM22-alpha homolog, Epididymis tissue protein Li 7e

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.4 kDa (208aa) confirmed by MALDI-TOF

TAGLN2, Transgelin-2, HA1756, KIAA0120, Transgelin 2, ATGP0938-10 µg, ATGP0938-20 µg, ATGP0938-50 µg, ATGP0938-100 µg, ATGP0938-250 µg, ATGP0938-500 µg, ATGP0938-1 mg, ATGP0938-10, ATGP0938-20, ATGP0938-50, ATGP0938-100, ATGP0938-250, ATGP0938-500, ATGP0938-1

Shapland C, et al. (1993) . J. Cell Biol., 121:1065-1073.; ; Kobayashi R, et al. (1994) . Biochem. Biophys. Res. Commun. 198:1275-1280.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Development

NP_003555

P37802

Human

EVQQKIEKQ YDADLEQILI QWITTQCRKD VGRPQPGREN FQNWLKDGTV LCELINALYP EGQAPVKKIQ ASTMAFKQME QISQFLQAAE RYGINTTDIF QTVDLWEGKN MACVQRTLMN LGGLAVARDD GLFSGDPNWF PKKSKENPRN FSDNQLQEGK NVIGLQMGTN RGASQAGMTG YGMPRQIL