Recombinant humaan transgeline 3/TAGLN3-eiwit

TAGLN3, also known as transgelin-3, contains a putative Actin-binding domain, two EF-hand motifs, two potential phosphorylation sites and a calponin-homology (CH) domain. It shares homology with transgelin and calponin, two cytoskeleton-interacting proteins. Belonging to the calponin family, TAGLN3 co-localizes with Actin and tubulin, suggesting a possible role for it in neuronal plasticity or as a signaling protein. Due to a varied expression pattern, it may play different roles in the developing and adult brain. Recombinant human TAGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Transgelin-3, NP22, NP25

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.6 kDa (219aa) confirmed by MALDI-TOF

TAGLN3, Transgelin-3, NP22, NP25, ATGP1234-10 µg, ATGP1234-20 µg, ATGP1234-50 µg, ATGP1234-100 µg, ATGP1234-250 µg, ATGP1234-500 µg, ATGP1234-1 mg, ATGP1234-10, ATGP1234-20, ATGP1234-50, ATGP1234-100, ATGP1234-250, ATGP1234-500, ATGP1234-1

Ren W.Z. et al. (1994) Brain Res.Mol. Brain Res. 22: 173-185.; ; Fan L. et al. (2001) J. Neurochem. 76: 1275-1281.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Neuroscience

NP_037391.2

Q9UI15

Human

MANRGPSYGL SREVQEKIEQ KYDADLENKL VDWIILQCAE DIEHPPPGRA HFQKWLMDGT VLCKLINSLY PPGQEPIPKI SESKMAFKQM EQISQFLKAA ETYGVRTTDI FQTVDLWEGK DMAAVQRTLM ALGSVAVTKD DGCYRGEPSW FHRKAQQNRR GFSEEQLRQG QNVIGLQMGS NKGASQAGMT GYGMPRQIM