Recombinant humaan transgeline/TAGLN-eiwit

TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein that belongs to the calponin family. This protein is expressed abundantly in fibroblasts and smooth muscle. It is involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. During embryogenesis, TAGLN is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. It is downregulated in several transformed cell lines, indicating that a reduction of TAGLN expression may be an early indicator of the onset of transformation. Recombinant human TAGLN protein, fused to his-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques

WS3-10, Transgelin, TAGLN1, Smooth muscle protein 22-alpha, SMCC, SM22 alpha, SM22

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.8 kDa (221aa) confirmed by MALDI-TOF

SM 22, SM22, SM22 alpha, SM22-alpha, SMCC, Smooth muscle protein 22 alpha, Smooth muscle protein 22-alpha, TAGLN, TAGLN 1, TAGLN1, TAGLN-1, Transgelin, WS3 10, WS310, WS3-10, ATGP0602-10 µg, ATGP0602-20 µg, ATGP0602-50 µg, ATGP0602-100 µg, ATGP0602-250 µg, ATGP0602-500 µg, ATGP0602-1 mg, ATGP0602-010, ATGP0602-020, ATGP0602-050, ATGP0602-100, ATGP0602-250, ATGP0602-500, ATGP0602-01M

Kobayashi R., et al. (1994) Biochem Biophys Res Commun. 198 (3) :1275-80. ; ; Thweatt R., et al. (1992) Biochem Biophys Res Commun. 187 (1) :1-7. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT

Cytoskeleton

NP_001001522

Q01995

Human

MANKGPSYGM SREVQSKIEK KYDEELEERL VEWIIVQCGP DVGRPDRGRL GFQVWLKNGV ILSKLVNSLY PDGSKPVKVP ENPPSMVFKQ MEQVAQFLKA AEDYGVIKTD MFQTVDLFEG KDMAAVQRTL MALGSLAVTK NDGHYRGDPN WFMKKAQEHK REFTESQLQE GKHVIGLQMG SNRGASQAGM TGYGRPRQII S