Recombinant humaan transglutaminase 2/TGM2-eiwit

TGM2 also known as Protein-glutamine gamma-glutamyltransferase 2. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Recombinant human TGM2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein-glutamine gamma-glutamyltransferase 2 isoform a, Transglutaminase 2, G-ALPHA-h, GNAH, HEL-S-45, TG2; TGC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

79.7 kDa (710aa)

Transglutaminase2, TGM2, Protein-glutamine gamma-glutamyltransferase 2 isoform a, Transglutaminase 2, G-ALPHA-h, GNAH, HEL-S-45, TG2; TGC, ATGP3003-10 µg, ATGP3003-20 µg, ATGP3003-50 µg, ATGP3003-100 µg, ATGP3003-250 µg, ATGP3003-500 µg, ATGP3003-1 mg, ATGP3003-10, ATGP3003-20, ATGP3003-50, ATGP3003-100, ATGP3003-250, ATGP3003-500, ATGP3003-1

Jeong JH., et al. (2013) J. Korean Med. Sci. 28 (7), 1005-1014

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Apoptosis

NP_004604

P21980

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEELVL ERCDLELETN GRDHHTADLC REKLVVRRGQ PFWLTLHFEG RNYEASVDSL TFSVVTGPAP SQEAGTKARF PLRDAVEEGD WTATVVDQQD CTLSLQLTTP ANAPIGLYRL SLEASTGYQG SSFVLGHFIL LFNAWCPADA VYLDSEEERQ EYVLTQQGFI YQGSAKFIKN IPWNFGQFED GILDICLILL DVNPKFLKNA GRDCSRRSSP VYVGRVVSGM VNCNDDQGVL LGRWDNNYGD GVSPMSWIGS VDILRRWKNH GCQRVKYGQC WVFAAVACTV LRCLGIPTRV VTNYNSAHDQ NSNLLIEYFR NEFGEIQGDK SEMIWNFHCW VESWMTRPDL QPGYEGWQAL DPTPQEKSEG TYCCGPVPVR AIKEGDLSTK YDAPFVFAEV NADVVDWIQQ DDGSVHKSIN RSLIVGLKIS TKSVGRDERE DITHTYKYPE GSSEEREAFT RANHLNKLAE KEETGMAMRI RVGQSMNMGS DFDVFAHITN NTAEEYVCRL LLCARTVSYN GILGPECGTK YLLNLNLEPF SEKSVPLCIL YEKYRDCLTE SNLIKVRALL VEPVINSYLL AERDLYLENP EIKIRILGEP KQKRKLVAEV SLQNPLPVAL EGCTFTVEGA GLTEEQKTVE IPDPVEAGEE VKVRMDLLPL HMGLHKLVVN FESDKLKAVK GFRNVIIGPA