Recombinant humaan Trappine-2/Elafin-eiwit

PI3 is an elastase-specific protease inhibitor, which contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. PI3 belongs to the centromeric cluster. Recombinant human PI3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Elafin preproprotein, Cementoin, ESI, SKALP, WAP3, WFDC14

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

12 kDa (118aa) confirmed by MALDI-TOF

Trappin2, Elafin, Elafin preproprotein, Cementoin, ESI, SKALP, WAP3, WFDC14, ATGP2244-10 µg, ATGP2244-20 µg, ATGP2244-50 µg, ATGP2244-100 µg, ATGP2244-250 µg, ATGP2244-500 µg, ATGP2244-1 mg, ATGP2244-10, ATGP2244-20, ATGP2244-50, ATGP2244-100, ATGP2244-250, ATGP2244-500, ATGP2244-1

Sumi Y, Inoue N, et al. (2002) . Atherosclerosis. 160 (1) :31-9.; ; Kuijpers AL, Pfundt R, et al. (1998) . Clin Genet. 54 (1) :96-101.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_002629

P19957

Human

AVTGVPV KGQDTVKGRV PFNGQDPVKG QVSVKGQDKV KAQEPVKGPV STKPGSCPII LIRCAMLNPP NRCLKDTDCP GIKKCCEGSC GMACFVPQ