Recombinant humaan TRDMT1-eiwit

TRDMT1 (tRNA aspartic acid methyltransferase 1), also known as DNMT2, specifically methylates cytosine 38 in the anticodon loop of tRNA (Asp) . Recombinant human TRDMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

TRNA (cytosine (38) -C (5) ) -methyltransferase, M.HsaIIP, PuMet, RNMT1, DNMT2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.1 kDa (415aa) confirmed by MALDI-TOF

TRDMT1, tRNA (cytosine (38) -C (5) ) -methyltransferase, M.HsaIIP, PuMet, RNMT1, DNMT2, ATGP1495-10 µg, ATGP1495-20 µg, ATGP1495-50 µg, ATGP1495-100 µg, ATGP1495-250 µg, ATGP1495-500 µg, ATGP1495-1 mg, ATGP1495-10, ATGP1495-20, ATGP1495-50, ATGP1495-100, ATGP1495-250, ATGP1495-500, ATGP1495-1

Okano M., et al. (1998) Nucleic Acids Res. 26:2536-2540; ; Franchina M., et al. (2001) Int. J. Biochem. Cell Biol. 33:1104-1115

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

AAH47733

O14717

Human

MEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE