Recombinant humaan TREM2-eiwit

TREM2 is a membrane protein that forms a receptorsignaling complex with the TYRO protein tyrosine kinase bindingprotein. The protein functions in immune response and maybe involved in chronic inflammation by triggering the production ofconstitutive inflammatory cytokines. Defects in this gene are acause of polycystic lipomembranous osteodysplasia with sclerosingleukoencephalopathy (PLOSL) . Alternative splicing results in multiple transcript variants encoding different isoforms. Recombinant human TREM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ttriggering receptor expressed on myeloid cells 2 isoform 1, Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on myeloid cells 2a, TREM-2, Trem2a, Trem2b, Trem2c

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.4 kDa (181aa) confirmed by MALDI-TOF

TREM2, ATGP2452-10 µg, ATGP2452-20 µg, ATGP2452-50 µg, ATGP2452-100 µg, ATGP2452-250 µg, ATGP2452-500 µg, ATGP2452-1 mg, ATGP2452-10, ATGP2452-20, ATGP2452-50, ATGP2452-100, ATGP2452-250, ATGP2452-500, ATGP2452-1

Bouchon A., et al (2000) . J. Immunol. 164:4991-4995

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Immunology

NP_061838.1

Q9NZC2

Human

HN TTVFQGVAGQ SLQVSCPYDS MKHWGRRKAW CRQLGEKGPC QRVVSTHNLW LLSFLRRWNG STAITDDTLG GTLTITLRNL QPHDAGLYQC QSLHGSEADT LRKVLVEVLA DPLDHRDAGD LWFPGESESF EDAHVEHSIS R