Recombinant humaan TRIAP1-eiwit

TP53-regulated inhibitor of apoptosis 1, also known as TRIAP1, contains a p53-binding site within its second exon and the reduction of expression by small interfering RNA enhanced apoptosis, whereas overexpression protected cells from apoptosis caused by DNA damage. TRIAP1 is induced significantly when cells have a low level of genotoxic stresses, but not when DNA damage is severe. TRIAP1 can modulate apoptotic pathways through interaction with Hsp70 that probably inhibits activity of apoptosis protease activating factor-1. Recombinant human TRIAP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TP53-regulated inhibitor of apoptosis 1, HSPC132, MDM35, P53CSV, WF-1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

11.2 kDa (99aa) confirmed by MALDI-TOF

TRIAP1, TP53-regulated inhibitor of apoptosis 1, HSPC132, MDM35, P53CSV, WF-1, ATGP2431-10 µg, ATGP2431-20 µg, ATGP2431-50 µg, ATGP2431-100 µg, ATGP2431-250 µg, ATGP2431-500 µg, ATGP2431-1 mg, ATGP2431-10, ATGP2431-20, ATGP2431-50, ATGP2431-100, ATGP2431-250, ATGP2431-500, ATGP2431-1

Park WR., et al. (2005) Cancer Res. 65 (4) : 1197-206.; ; Staib F., et al. (2005) Cancer Res. 65 (22) : 10255-64.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Apoptosis

NP_057483

O43715

Human

MNSVGEA CTDMKREYDQ CFNRWFAEKF LKGDSSGDPC TDLFKRYQQC VQKAIKEKEI PIEGLEFMGH GKEKPENSS