Recombinant humaan Trombomoduline/BDCA-3 eiwit

Thrombomodulin, also known as CD141 or BDCA-3, is an endothelial cell-expressed, transmembrane glycoprotein that can form a complex with the coagulation factor, thrombin. This complex induce activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI) into its active form. Reduced levels of thrombomodulin or increased serum levels of THBD can correlate with pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. In addition, It associated with liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis. Recombinant human Thrombomodulin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

THBD, Thrombomodulin, TM, Fetomodulin, CD141, CD141 antigen, THRM, BDCA-3, BDCA3, blood dendritic cell antigen 3, AHUS6, THPH12

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

52.6kDa (500aa)

, ATGP3996-10 µg, ATGP3996-20 µg, ATGP3996-50 µg, ATGP3996-100 µg, ATGP3996-250 µg, ATGP3996-500 µg, ATGP3996-1 mg, ATGP3996-10, ATGP3996-20, ATGP3996-50, ATGP3996-100, ATGP3996-250, ATGP3996-500, ATGP3996-1

Bajzar L, Morser J, Nesheim M (1996) . The Journal of Biological Chemistry. 271: 16603–16608.; ; Jakubowski HV, Owen WG (1989) . The Journal of Biological Chemistry. 264: 11117–11121.; ; Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515-1524.; ; Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59-66.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000352

P07204

Human

Glu22