Recombinant humaan Trombopoëtine-eiwit

Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Thrombopoietin fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/mL.

THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO, thrombopoietin isoform 1 precursor, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ATGP3057

HEK293

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤10 ng/mL.

36.8kDa (343aa)

, ATGP3970-10 µg, ATGP3970-20 µg, ATGP3970-50 µg, ATGP3970-100 µg, ATGP3970-250 µg, ATGP3970-500 µg, ATGP3970-1 mg, ATGP3970-10, ATGP3970-20, ATGP3970-50, ATGP3970-100, ATGP3970-250, ATGP3970-500, ATGP3970-1

Fishley B, et al, (2004) Growth Factors. 22:151-155.; ; Chou FS, et al, (2011) J Cell Biochem. 112:1491-1498.; ; Kong Z, et al, (2017) Blood. 130:1097-1103.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000451.1

P40225

Human

Asp

SPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEG