Recombinant humaan Troponine T-eiwit

Troponin T, also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Troponin T type 2 cardiac isoform 3, Troponin T type 2, cardiac isoform 3, CMH2, CMPD2, cTnT, RCM3, TnTC, Troponin T type 2, cardiac isoform 3 Cardiac muscle troponin T, MGC3889, TNNT 2, TNNT2, Cardiomyopathy hypertrophic 2, Cardiomyopathy dilated 1D (autosomal dominant), CMD1D, Troponin T cardiac muscle, Troponin T type 2 (cardiac), Troponin T type 2 cardiac, Troponin T2 cardiac.

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.4 kDa (305aa) confirmed by MALDI-TOF

Troponin T, Troponin T type 2 cardiac isoform 3, Troponin T type 2, cardiac isoform 3, CMH2, CMPD2, cTnT, RCM3, TnTC, Troponin T type 2, cardiac isoform 3 Cardiac muscle troponin T, MGC3889, TNNT 2, TNNT2, Cardiomyopathy hypertrophic 2, Cardiomyopathy dilated 1D (autosomal dominant), CMD1D, Troponin T cardiac muscle, Troponin T type 2 (cardiac), Troponin T type 2 cardiac, Troponin T2 cardiac, ATGP0329-100, ATGP0329-10 µg, ATGP0329-20 µg, ATGP0329-50 µg, ATGP0329-100 µg, ATGP0329-250 µg, ATGP0329-500 µg, ATGP0329-1 mg, ATGP0329-10, ATGP0329-20, ATGP0329-50, ATGP0329-100, ATGP0329-250, ATGP0329-500, ATGP0329-1

Yu H., et al. (2009), Proteomics. 9 (3) :504-11.; ; Morita H., et al. (2008) . N Engl J Med. 358 (18) :1899-908.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.5M NaCl, 0.1mM PMSF, 1mM DTT, 100 mM imidazole

Cytoskeleton

NP_001001431.1

P45379

Human

MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK