Recombinant humaan TRPT1-eiwit

TRPT1, also known as tRNA 2'-phosphotransferase 1, catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate. Recombinant human TRPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

TRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.3 kDa (277aa) confirmed by MALDI-TOF

TRPT1, tRNA 2'-phosphotransferase 1, MGC11134, tRNA phosphotransferase 1, ATGP1327-10 µg, ATGP1327-20 µg, ATGP1327-50 µg, ATGP1327-100 µg, ATGP1327-250 µg, ATGP1327-500 µg, ATGP1327-1 mg, ATGP1327-10, ATGP1327-20, ATGP1327-50, ATGP1327-100, ATGP1327-250, ATGP1327-500, ATGP1327-1

Hu Q.-D., et al. (2003) Cell. Mol. Life Sci. 60:1725-1732; ; Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Enzymes & Proteases

NP_001153862

Q86TN4

Human

MNFSGG GRQEAAGSRG RRAPRPREQD RDVQLSKALS YALRHGALKL GLPMGADGFV PLGTLLQLPQ FRGFSAEDVQ RVVDTNRKQR FALQLGDPST GLLIRANQGH SLQVPKLELM PLETPQALPP MLVHGTFWKH WPSILLKGLS CQGRTHIHLA PGLPGDPGII SGMRSHCEIA VFIDGPLALA DGIPFFRSAN GVILTPGNTD GFLLPKYFKE ALQLRPTRKP LSLAGDEETE CQSSPKHSSR ERRRIQQ