Recombinant humaan Tryptase alfa/beta-1-eiwit

TPSAB1, also known as tryptase alpha/beta-1, is a serine protease with trypsin-like specificity. It is the key neutral protease present in mast cells and is discharged upon the coupled activation-degranulation response of this cell type. This protein is enzymatically active only as a heparin-stabilized tetramer, and is resistant to all known endogenous proteinase inhibitors. Also, it is implicated as a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

28.2 kDa (251aa)

Tryptase b2, Tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1, ATGP3688-10 µg, ATGP3688-20 µg, ATGP3688-50 µg, ATGP3688-100 µg, ATGP3688-250 µg, ATGP3688-500 µg, ATGP3688-1 mg, ATGP3688-10, ATGP3688-20, ATGP3688-50, ATGP3688-100, ATGP3688-250, ATGP3688-500, ATGP3688-1

Schwartz, L.B., et al, (1985) J. Immunol. 135:2762-2767.; ; Lewicki L., et al, (2015) Mediators Inflamm. 2015:395173.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Immunology

NP_003285.2

Q15661

Human

Ile31