Recombinant humaan Tryptase bèta-2-eiwit

TPSAB1, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human TPSAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Tryptase alpha/beta-1, TPS1, TPS2, TPSB1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

30.1 kDa (270aa)

Tryptase b2, Tryptase alpha/beta-1, TPS1, TPS2, TPSB1, ATGP1729-10 µg, ATGP1729-20 µg, ATGP1729-50 µg, ATGP1729-100 µg, ATGP1729-250 µg, ATGP1729-500 µg, ATGP1729-1 mg, ATGP1729-10, ATGP1729-20, ATGP1729-50, ATGP1729-100, ATGP1729-250, ATGP1729-500, ATGP1729-1

Tanaka T. et al. (1983) J Biol Chem. 258 (22) :13552-7.; ; Marquardt u. et al. (2002) J Mol Biol. 321 (3) :491-502.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Metabolism

NP_003285.2

Q15661

Human

IVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP