Recombinant humaan TSFM-eiwit

TSFM is a mitochondrial translation elongation factor. It is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in TSFM are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants. Recombinant human TSFM protein, fused to His-tag at N-terminus, was expressed in E. coli.

Ts translation elongation factor mitochondrial, Ts translation elongation factor, mitochondrial, COXPD3, EF-TS, EF-Tsmt

E.coli

Human

Non-Tagged

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.9 kDa (302aa)

TSFM, Ts translation elongation factor mitochondrial, Ts translation elongation factor, mitochondrial, COXPD3, EF-TS, EF-Tsmt, ATGP2691-10 µg, ATGP2691-20 µg, ATGP2691-50 µg, ATGP2691-100 µg, ATGP2691-250 µg, ATGP2691-500 µg, ATGP2691-1 mg, ATGP2691-10, ATGP2691-20, ATGP2691-50, ATGP2691-100, ATGP2691-250, ATGP2691-500, ATGP2691-1

Xin H. et al. (1995) J Biol Chem. 270:17243-17249.; ; Smeitink JA. et al. (2006) Am. J. Hum. Genet. 79:869-877.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Epigenomics (Transcription & Translation)

NP_005717.2

P43897

Human

MSKELLMKLR RKTGYSFVNC KKALETCGGD LKQAEIWLHK EAQKEGWSKA AKLQGRKTKE GLIGLLQEGN TTVLVEVNCE TDFVSRNLKF QLLVQQVALG TMMHCQTLKD QPSAYSKVQW LTPVNLALWE AEAGGSLEGF LNSSELSGLP AGPDREGSLK DQLALAIGKL GENMILKRAA WVKVPSGFYV GSYVHGAMQS PSLHKLVLGK YGALVICETS EQKTNLEDVG RRLGQHVVGM APLSVGSLDD EPGGEAETKM LSQPYLLDPS ITLGQYVQPQ GVSVVDFVRF ECGEGEEAAE TE