Recombinant humaan TSTA3-eiwit

TSTA3 is a NADP (H) -binding protein. It catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. Recombinant human TSTA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GDP-L-fucose synthase, FX, P35B, SDR4E1, GDP-L-fucose synthase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

38.0 kDa (341aa) confirmed by MALDI-TOF

TSTA3, GDP-L-fucose synthase, FX, P35B, SDR4E1, GDP-L-fucose synthase, ATGP0657-10 µg, ATGP0657-20 µg, ATGP0657-50 µg, ATGP0657-100 µg, ATGP0657-250 µg, ATGP0657-500 µg, ATGP0657-1 mg, ATGP0657-10, ATGP0657-20, ATGP0657-50, ATGP0657-100, ATGP0657-250, ATGP0657-500, ATGP0657-1

Camardella L., et al. (1995) Blood 85 (1) : 264-7; ; Tonetti M., et al. (1996) J. Biol. Chem. 271:27274-27279;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 50 mM NaCl

Metabolism

NP_003304

Q13630

Human

MGSSHHHHHH SSGLVPRGSH MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K