Recombinant humaan TTC1-eiwit

TTC1, also known as tetratricopeptide repeat protein 1, is composed of 292 amino acids with three TPR motifs, as a Galpha16-binding protein. The interaction was confirmed both in vitro and in transfected mammalian cells, where TTC1 also binds to several other Galpha proteins. TTC1 was found to interact with Ha-Ras preferentially in its active form. Recombinant human TTC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Tetratricopeptide repeat protein 1, TPR1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.1 kDa (316aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

TTC1, Tetratricopeptide repeat protein 1, TPR1, ATGP1641-10 µg, ATGP1641-20 µg, ATGP1641-50 µg, ATGP1641-100 µg, ATGP1641-250 µg, ATGP1641-500 µg, ATGP1641-1 mg, ATGP1641-10, ATGP1641-20, ATGP1641-50, ATGP1641-100, ATGP1641-250, ATGP1641-500, ATGP1641-1

Liu AM., et al. (2011) BMC Struct Biol. 13; ;11:17; ; Strausberg RL., et al. (2007) Proc. Natl. Acad. Sci. u.S.A. 26:16899-16903

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_003305

Q99614

Human

MGEKSE NCGVPEDLLN GLKVTDTQEA ECAGPPVPDP KNQHSQSKLL RDDEAHLQED QGEEECFHDC SASFEEEPGA DKVENKSNED VNSSELDEEY LIELEKNMSD EEKQKRREES TRLKEEGNEQ FKKGDYIEAE SSYSRALEMC PSCFQKERSI LFSNRAAARM KQDKKEMAIN DCSKAIQLNP SYIRAILRRA ELYEKTDKLD EALEDYKSIL EKDPSIHQAR EACMRLPKQI EERNERLKEE MLGKLKDLGN LVLRPFGLST ENFQIKQDSS TGSYSINFVQ NPNNNR