Recombinant humaan TTC32 eiwit

TTC32 contains 3 TPR repeats. The tetratricopeptide repeat (TPR) is a structural motif. It consists in a degenerate 34 amino acid sequence motif identified in a wide variety of proteins. TPR is found in tandem arrays of 3-16 motifs, which form scaffolds to mediate protein-protein interactions and often the assembly of multiprotein complexes. TPR-containing proteins include the anaphase-promoting complex (APC) subunits cdc16, cdc23 and cdc27, the NADPH oxidase subunit p67-phox, hsp90-binding immunophilins, transcription factors, the PKR protein kinase inhibitor, the major receptor for peroxisomal matrix protein import PEX5 and mitochondrial import proteins. Recombinant human TTC32 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tetratricopeptide repeat domain 32, TPR repeat protein 32, Tetratricopeptide repeat protein 32

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19.7 kDa (174aa) confirmed by MALDI-TOF

TTC32, tetratricopeptide repeat domain 32, TPR repeat protein 32, Tetratricopeptide repeat protein 32, ATGP1750-10 µg, ATGP1750-20 µg, ATGP1750-50 µg, ATGP1750-100 µg, ATGP1750-250 µg, ATGP1750-500 µg, ATGP1750-1 mg, ATGP1750-10, ATGP1750-20, ATGP1750-50, ATGP1750-100, ATGP1750-250, ATGP1750-500, ATGP1750-1

The MGC Project Team. (2004) Genome Res. 14:2121-2127

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol,2mM DTT

Other

NP_001008238

Q5I0X7

Human

MGSSHHHHHH SSGLVPRGSH MGSMEGQRQE SHATLTLAQA HFNNGEYAEA EALYSAYIRR CACAASSDES PGSKCSPEDL ATAYNNRGQI KYFRVDFYEA MDDYTSAIEV QPNFEVPYYN RGLILYRLGY FDDALEDFKK VLDLNPGFQD ATLSLKQTIL DKEEKQRRNV AKNY