Recombinant humaan TUBG1-eiwit

TuBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. Recombinant human TuBG1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

53.3 kDa (471aa)

TUBG1, Tubulin gamma-1 chain, GCP-1, TuBG, TuBGCP1, ATGP1295-10 µg, ATGP1295-20 µg, ATGP1295-50 µg, ATGP1295-100 µg, ATGP1295-250 µg, ATGP1295-500 µg, ATGP1295-1 mg, ATGP1295-10, ATGP1295-20, ATGP1295-50, ATGP1295-100, ATGP1295-250, ATGP1295-500, ATGP1295-1

Zheng Y., et al. (1991) Cell. 65 (5) :817-23.; ; Wise DO., et al. (2000) Genomics. 67 (2) :164-70.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 1M urea, 5% glycerol

Cytoskeleton

NP_001061

P23258

Human

MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q