Recombinant humaan TUBG1-eiwit

TUBG1, also known as tubulin gamma-1 chain, is a member of the tubulin superfamily. This protein is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. It is the pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. It is required for microtubule formation and progression of the cell cycle. Also, this protein is interacts with GCP2, GCP3, and B9D2. The interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Recombinant human TUBG1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tubulin gamma-1 chain, TUBG1, CDCBM4, GCP-1, TUBG, TUBGCP1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

51.9 kDa (457aa)

TUBG1, Tubulin gamma-1 chain, TUBG1, CDCBM4, GCP-1, TUBG, TUBGCP1, ATGP3767-10 µg, ATGP3767-20 µg, ATGP3767-50 µg, ATGP3767-100 µg, ATGP3767-250 µg, ATGP3767-500 µg, ATGP3767-1 mg, ATGP3767-10, ATGP3767-20, ATGP3767-50, ATGP3767-100, ATGP3767-250, ATGP3767-500, ATGP3767-1

Zheng Y., et al, (1991) Cell 65:817-823.; ; Kapeller R., et al, (1995) J. Biol. Chem. 270:25985-25991.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 2mM DTT, 50 mM imidazole.

Signal Transduction

NP_001061

P23258

Human

Met1