Recombinant humaan TYW5-eiwit

TRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7- (a-amino-a-carboxypropyl) wyosine (yW-72) into undermodified hydroxywybutosine (OHyW) . OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

38.9 kDa (338aa) confirmed by MALDI-TOF

TYW5, tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5, ATGP1851-10 µg, ATGP1851-20 µg, ATGP1851-50 µg, ATGP1851-100 µg, ATGP1851-250 µg, ATGP1851-500 µg, ATGP1851-1 mg, ATGP1851-10, ATGP1851-20, ATGP1851-50, ATGP1851-100, ATGP1851-250, ATGP1851-500, ATGP1851-1

Noma A., et al. (2010) J. Biol. Chem. 285:34503-34507; ; Kato M., et al. (2011) Nucleic Acids Res. 39:1576-1585;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_001034782

A2RUC4

Human

MAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE