Recombinant humaan U2AF1-eiwit

U2AF1 belongs to the splicing factor SR family of genes. u2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of u2 snRNP to the pre-mRNA branch site. This protein is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Recombinant human u2AF1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

U2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.3 kDa (263aa)

U2AF1, u2 small nuclear RNA auxiliary factor 1, FP793, RN, RNu2AF1, Splicing factor u2AF 35 kDa subunit, u2 auxiliary factor 35 kDa subunit, RN, RNu2AF1, u2AF35, u2AFBP, ATGP2095-10 µg, ATGP2095-20 µg, ATGP2095-50 µg, ATGP2095-100 µg, ATGP2095-250 µg, ATGP2095-500 µg, ATGP2095-1 mg, ATGP2095-10, ATGP2095-20, ATGP2095-50, ATGP2095-100, ATGP2095-250, ATGP2095-500, ATGP2095-1

Thol, F., et al. (2012) Blood 119 (15), 3578-3584; ; Makishima, H., et al. (2012) Blood 119 (14), 3203-3210

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Epigenomics (Transcription & Translation)

NP_001020374

Q01081

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEYLAS IFGTEKDKVN CSFYFKIGAC RHGDRCSRLH NKPTFSQTIL IQNIYRNPQN SAQTADGSHC AVSDVEMQEH YDEFFEEVFT EMEEKYGEVE EMNVCDNLGD HLVGNVYVKF RREEDAEKAV IDLNNRWFNG QPIHAELSPV TDFREACCRQ YEMGECTRGG FCNFMHLKPI SRELRRELYG RRRKKHRSRS RSRERRSRSR DRGRGGGGGG GGGGGGRERD RRRSRDRERS GRF