Recombinant humaan UBA5-eiwit

UBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-like modifier-activating enzyme 5, THIFP1, uBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

47.4 kDa (428aa) confirmed by MALDI-TOF

UFM1-activating enzyme, UFM1 Activating Enzyme, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1, uBE1DC1, UBA5, THIFP1, Ubiquitin like modifier-activating enzyme 5, Ubiquitin activating enzyme E1 domain containing protein 1, Ubiquitin like modifier activating enzyme 5, ATGP1507-10 µg, ATGP1507-20 µg, ATGP1507-50 µg, ATGP1507-100 µg, ATGP1507-250 µg, ATGP1507-500 µg, ATGP1507-1 mg, ATGP1507-010, ATGP1507-020, ATGP1507-050, ATGP1507-100, ATGP1507-250, ATGP1507-500, ATGP1507-01M

Komatsu M., et al. (2004) EMBO J. 23:1977-1986; ; Zheng M., et al. (2008) J. Cell. Biochem. 104:2324-2334

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Ubiquitination

NP_079094

Q9GZZ9

Human

MAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM