Recombinant humaan Ubc12/UBE2M-eiwit

UBE2M, also known as NEDD8-conjugating enzyme ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Recombinant human uBE2M protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M, Homologous to yeast UBC12, Ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), hUbc12, UBC12

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.5 kDa (207aa) confirmed by MALDI-TOF

UBE2M, Ubc12, NEDD8-conjugating enzyme ubc12, hubc12, uBC-RS2, uBC12, ATGP1603-10 µg, ATGP1603-20 µg, ATGP1603-50 µg, ATGP1603-100 µg, ATGP1603-250 µg, ATGP1603-500 µg, ATGP1603-1 mg, ATGP1603-10, ATGP1603-20, ATGP1603-50, ATGP1603-100, ATGP1603-250, ATGP1603-500, ATGP1603-1

Gong L., et al. (1999) J. Biol. Chem. 274:12036-12042; ; Huang D.T., et al. (2004) Nat. Struct. Mol. Biol. 11:927-935

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.2M NaCl, 1mM DTT

Ubiquitination

NP_003960

P61081

Human

MGSSHHHHHH SSGLVPRGSH MGSHMIKLFS LKQQKKEEES AGGTKGSSKK ASAAQLRIQK DINELNLPKT CDISFSDPDD LLNFKLVICP DEGFYKSGKF VFSFKVGQGY PHDPPKVKCE TMVYHPNIDL EGNVCLNILR EDWKPVLTIN SIIYGLQYLF LEPNPEDPLN KEAAEVLQNN RRLFEQNVQR SMRGGYIGST YFERCLK