Recombinant humaan Ubc13/UBE2N-eiwit

UBE2N, also known as UBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage. Recombinant human UBE2N protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, E2 ubiquitin-conjugating enzyme N, Ubc13, UbcH13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, BLU

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19.3 kDa (172aa) confirmed by MALDI-TOF

UBE2N, ATGP0993-10 µg, ATGP0993-20 µg, ATGP0993-50 µg, ATGP0993-100 µg, ATGP0993-250 µg, ATGP0993-500 µg, ATGP0993-1 mg, ATGP0993-10, ATGP0993-20, ATGP0993-50, ATGP0993-100, ATGP0993-250, ATGP0993-500, ATGP0993-1

Yamaguchi T. et al. (1996) J Biochem., 120:494-497.; ; Yamamoto M. et al. (2006) Nat. Immunol. 7: 962-970.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Ubiquitination

NP_003339

P61088

Human

MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI