Recombinant humaan Ubc2/UBE2A-eiwit

Ubiquitin-conjugating enzyme E2 A isoform 1, also known as uBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. Recombinant human uBE2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 A, RAD6 homolog A, RAD6A, HR6A, hHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, UBC2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19.4 kDa (172aa) confirmed by MALDI-TOF

UBE2A, ubiquitin-conjugating enzyme E2 A isoform 1, HHR6A, RAD6A, uBC2, ubiquitin-conjugating enzyme E2 A isoform 1 BHR6A, hHR6A, HR6A, mHR6A, uBCD6, uBE2A, ubiquitin carrier protein, ubiquitin conjugating enzyme E2 17 kDa, ubiquitin conjugating enzyme E2 21.5 kDa, ubiquitin conjugating enzyme E2 A, ubiquitin protein ligase A., ATGP0555-10 µg, ATGP0555-20 µg, ATGP0555-50 µg, ATGP0555-100 µg, ATGP0555-250 µg, ATGP0555-500 µg, ATGP0555-1 mg, ATGP0555-10, ATGP0555-20, ATGP0555-50, ATGP0555-100, ATGP0555-250, ATGP0555-500, ATGP0555-1

Sarcevic B., et al. (2002) EMBO J. 21 (8) :2009-18.; ; Ciechanover A., et al. (1994) FASEB. 8:182-191.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA

Ubiquitination

NP_003327

P49459

Human

MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC