Recombinant humaan Ubc2/UBE2B eiwit

UBE2B, also known as ubiquitin-conjugating enzyme E2 B, belongs to E2 ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins, and associates to the E3 ligase RAD18 to form the uBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. It is required for postreplication repair of uV-damaged DNA. It may be involved in neurite outgrowth. Recombinant human uBE2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 B, E2 ubiquitin-conjugating enzyme B, RAD6 homolog B, RAD6B HR6B, hHR6B, Ubiquitin carrier protein B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B, Ubc2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.4 kDa (188aa) confirmed by MALDI-TOF

E2 17 kDa, E2 17kDa, E2 ubiquitin conjugating enzyme B, E2 ubiquitin-conjugating enzyme B, E2-17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, RAD6B HR6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2 17 kDa, Ubiquitin conjugating enzyme E2 17kDa, Ubiquitin conjugating enzyme E2 B, Ubiquitin conjugating enzyme E2B, Ubiquitin conjugating enzyme E2B hHR6B, Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa, Ubiquitin-conjugating enzyme E2 17kDa, Ubiquitin-conjugating enzyme E2 B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-conjugating enzyme E2-17kDa, Ubiquitin-conjugating enzyme E2B, Ubiquitin-conjugating enzyme E2B hHR6B, Ubiquitin-protein ligase B, ATGP0569-10 µg, ATGP0569-20 µg, ATGP0569-50 µg, ATGP0569-100 µg, ATGP0569-250 µg, ATGP0569-500 µg, ATGP0569-1 mg, ATGP0569-010, ATGP0569-020, ATGP0569-050, ATGP0569-100, ATGP0569-250, ATGP0569-500, ATGP0569-01M

Koken MH. et al. (1996) Dev Biol. 173 (1) :119-32.; ; Xin H, et al. (2000) Nucleic Acids Res.. 28 (14) :2847-54;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Ubiquitination

NP_003328

P63146

Human

MSTP ARRRLMRDFK RLQEDPPVGV SGAPSENNIM QWNAVIFGPE GTPFEDGTFK LVIEFSEEYP NKPPTVRFLS KMFHPNVYAD GSICLDILQN RWSPTYDVSS ILTSIQSLLD EPNPNSPANS QAAQLYQENK REYEKRVSAI VEQSWNDS