Recombinant humaan UBC3B/UBE2R2-eiwit

UBE2R2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This protein is a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. Recombinant human UBE2R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 R2, E2 ubiquitin-conjugating enzyme R2, Ubiquitin carrier protein R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, CDC34B, UBC3B

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.6 kDa (261aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CDC34B, E2 CDC34B, E2 ubiquitin conjugating enzyme R2, E2 ubiquitin-conjugating enzyme R2, E2CDC34B, E2-CDC34B, UBC3B, UBE2R2, Ubiquitin carrier protein R2, Ubiquitin conjugating enzyme E2 R2, Ubiquitin conjugating enzyme E2-CDC34B, Ubiquitin protein ligase R2, Ubiquitin-conjugating enzyme E2 R2, Ubiquitin-conjugating enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin conjugating enzyme E2 CDC34B, Ubiquitin-conjugating enzyme E2 CDC34B, ATGP2374-10 µg, ATGP2374-20 µg, ATGP2374-50 µg, ATGP2374-100 µg, ATGP2374-250 µg, ATGP2374-500 µg, ATGP2374-1 mg, ATGP2374-010, ATGP2374-020, ATGP2374-050, ATGP2374-100, ATGP2374-250, ATGP2374-500, ATGP2374-01M

Yoshida, T., et al. (2010) Int. J. Mol. Med. 25 (4), 649-656; ; Oguri, M., et al. (2006) Am. J. Hypertens. 23 (1), 70-77;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_060281

Q712K3

Human

MAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S