Recombinant humaan Ubc6/UBE2J2-eiwit

UBE2J2, also known as ubiquitin-conjugating enzyme E2 J2, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Recombinant human uBE2J2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Uubiquitin-conjugating enzyme E2 J2 isoform 2, Ubiquitin-conjugating enzyme E2, J2, UBC6 homolog, yeast, Ubc6p, UBC6, NCUBE2, E2 ubiquitin-conjugating enzyme J2, Non-canonical ubiquitin-conjugating enzyme 2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

28kDa (250aa) confirmed by MALDI-TOF

UBE2J2, ubiquitin-conjugating enzyme E2 J2, NCuBE-2, NCuBE2, PRO2121, ATGP1608-10 µg, ATGP1608-20 µg, ATGP1608-50 µg, ATGP1608-100 µg, ATGP1608-250 µg, ATGP1608-500 µg, ATGP1608-1 mg, ATGP1608-10, ATGP1608-20, ATGP1608-50, ATGP1608-100, ATGP1608-250, ATGP1608-500, ATGP1608-1

Wang X, et al. (2009) J Cell Biol. 187 (5) :655-68.; ; Nandi, D. et al. (2006) Journal of biosciences 31 (1) : 137-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 1mM DTT, 200 mM NaCl

Ubiquitination

NP_477515

Q8N2K1

Human

MSSTSS KRAPTTATQR LKQDYLRIKK DPVPYICAEP LPSNILEWHY VVRGPEMTPY EGGYYHGKLI FPREFPFKPP SIYMITPNGR FKCNTRLCLS ITDFHPDTWN PAWSVSTILT GLLSFMVEKG PTLGSIETSD FTKRQLAVQS LAFNLKDKVF CELFPEVVEE IKQKQKAQDE LSSRPQTLPL PDVVPDGETH LVQNGIQLLN GHAPGAVPNL AGLQQANRHH