Recombinant humaan UbcH10/UBE2C-eiwit

Ubiquitin-conjugating enzyme E2 C, also known as uBE2C, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2C protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 C, E2 ubiquitin-conjugating enzyme C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.1 kDa (202aa) confirmed by MALDI-TOF

UbcH10, UBE2C, ubiquitin-conjugating enzyme E2 C, dJ447F3.2; uBCH10, ATGP1681-10 µg, ATGP1681-20 µg, ATGP1681-50 µg, ATGP1681-100 µg, ATGP1681-250 µg, ATGP1681-500 µg, ATGP1681-1 mg, ATGP1681-10, ATGP1681-20, ATGP1681-50, ATGP1681-100, ATGP1681-250, ATGP1681-500, ATGP1681-1

Okamoto Y., et al. (2003) Cancer Res. 63:4167-4173; ; Wagner K.W., et al. (2004) Oncogene. 23:6621-6629

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Ubiquitination

NP_008950

O00762

Human

MASQNRD PAATSVAAAR KGAEPSGGAA RGPVGKRLQQ ELMTLMMSGD KGISAFPESD NLFKWVGTIH GAAGTVYEDL RYKLSLEFPS GYPYNAPTVK FLTPCYHPNV DTQGNICLDI LKEKWSALYD VRTILLSIQS LLGEPNIDSP LNTHAAELWK NPTAFKKYLQ ETYSKQVTSQ EP