Recombinant humaan UbcH2/UBE2H-eiwit

UBE2H (ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. uBE2H is Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2H protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 H, E2 ubiquitin-conjugating enzyme H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBCH, UBC8, GID3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.1 kDa (206aa) confirmed by MALDI-TOF

UbcH2, UBE2H, ubiquitin-conjugating enzyme E2H, E2-20K, uBC8, uBCH, uBCH2, ATGP1508-10 µg, ATGP1508-20 µg, ATGP1508-50 µg, ATGP1508-100 µg, ATGP1508-250 µg, ATGP1508-500 µg, ATGP1508-1 mg, ATGP1508-10, ATGP1508-20, ATGP1508-50, ATGP1508-100, ATGP1508-250, ATGP1508-500, ATGP1508-1

Kaiser P., et al. (1994) J. Biol. Chem. 269:8797-8802; ; David Y., et al. (2010) J. Biol. Chem. 285:8595-8604

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Ubiquitination

NP_003335

P62256

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL