Recombinant humaan UbcH5a/UBE2D1-eiwit

UBE2D1 belongs to the ubiquitin-conjugating enzyme family. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. uBE2D1 is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Recombinant human uBE2D1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ubiquitin conjugating enzyme E2 D1, SFT, Stimulator of Fe transport, Ubiquitin-conjugating enzyme E2D 1, "UBC4/5 homolog, yeast", UbcH5A, UBCH5, UBC4/5, E2 (17) KB1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

19 kDa (170aa) confirmed by MALDI-TOF

UbcH5a, UBE2D1, ubiquitin-conjugating enzyme E2D 1, SFT, uBC5A, uBCH5, uBCH5A, ATGP1074-10 µg, ATGP1074-20 µg, ATGP1074-50 µg, ATGP1074-100 µg, ATGP1074-250 µg, ATGP1074-500 µg, ATGP1074-1 mg, ATGP1074-10, ATGP1074-20, ATGP1074-50, ATGP1074-100, ATGP1074-250, ATGP1074-500, ATGP1074-1

Windheim M., et al. (2008) Biochem. J. 409:723-729; ; Pabarcus M.K., et al. (2009) Arch. Biochem. Biophys 483:66-74

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl

Ubiquitination

NP_003329

P51668

Human

MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM