Recombinant humaan UbcH5b/UBE2D2-eiwit

UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. uBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human uBE2D2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2 (17) KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.9 kDa (167aa) confirmed by MALDI-TOF

UbcH5b, UBE2D2, ubiquitin-conjugating enzyme E2 D2, E2 (17) KB2, PuBC1, uBC4, uBC4/5, uBCH5B, ATGP1147-10 µg, ATGP1147-20 µg, ATGP1147-50 µg, ATGP1147-100 µg, ATGP1147-250 µg, ATGP1147-500 µg, ATGP1147-1 mg, ATGP1147-10, ATGP1147-20, ATGP1147-50, ATGP1147-100, ATGP1147-250, ATGP1147-500, ATGP1147-1

Ciechanover A., et al. (1994) FASEB J. 8 (2) :182-19.; ; Orian A., et al. (1995) J Biol Chem. 270 (37) :21707-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Ubiquitination

NP_003330

P62837

Human

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM