Recombinant humaan UbcH5b/UBE2D2-eiwit

UBE2D2, also known as Ubiquitin-conjugating enzyme E2 D2, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner. Recombinant human UBE2D2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2 (17) KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B

E.coli

Human

Non-Tagged

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

16.7 kDa (147aa) Confirmed by MALDI-TOF

UbcH5b, UBE2D2, Ubiquitin-conjugating enzyme E2 D2 isoform1, E2 (17) KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B, ATGP3690-10 µg, ATGP3690-20 µg, ATGP3690-50 µg, ATGP3690-100 µg, ATGP3690-250 µg, ATGP3690-500 µg, ATGP3690-1 mg, ATGP3690-10, ATGP3690-20, ATGP3690-50, ATGP3690-100, ATGP3690-250, ATGP3690-500, ATGP3690-1

Ciechanover A., et al. (1994) FASEB J. 8 (2) :182-91.; ; Orian A., et al. (1995) J Biol Chem. 270 (37) :21707-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 6.0) containing 50 mM NaCl, 1mM DTT

Ubiquitination

NP_003330

P62837

Human

MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM