Recombinant humaan UbcH5c/UBE2D3-eiwit

UBE2D3, also known as ubiquitin-conjugating enzyme E2 D3. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Recombinant human UBE2D3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2 (17) KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3, UBC5C, UBCH5C

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

19 kDa (169aa) confirmed by MALDI-TOF

UbcH5UBE2D3, ubiquitin-conjugating enzyme E2 D3, uBC5C, uBCH5C, ATGP1270-10 µg, ATGP1270-20 µg, ATGP1270-50 µg, ATGP1270-100 µg, ATGP1270-250 µg, ATGP1270-500 µg, ATGP1270-1 mg, ATGP1270-10, ATGP1270-20, ATGP1270-50, ATGP1270-100, ATGP1270-250, ATGP1270-500, ATGP1270-1

Gonen H., et al. (1999) J. Biol. Chem. 274:14823-14830; ; Murata S., et al. (2001) EMBO Rep. 2:1133-1138

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Ubiquitination

NP_871622

P61077

Human

MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM