Recombinant humaan UbcH5d/UBE2D4-eiwit

UBE2D4 belongs to the ubiquitin-conjugating enzyme family. ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human uBE2D4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 D4, E2 ubiquitin-conjugating enzyme D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.8 kDa (167aa) confirmed by MALDI-TOF

UBE2D4, ubiquitin-conjugating enzyme E2 D4, uBCH5D, HBuCE1, ATGP1243-10 µg, ATGP1243-20 µg, ATGP1243-50 µg, ATGP1243-100 µg, ATGP1243-250 µg, ATGP1243-500 µg, ATGP1243-1 mg, ATGP1243-10, ATGP1243-20, ATGP1243-50, ATGP1243-100, ATGP1243-250, ATGP1243-500, ATGP1243-1

David Y., et al. (2010) J. Biol. Chem. 285:8595-8604; ; Nandi, D, et al. (2006) Journal of biosciences 31 (1) : 137-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Ubiquitination

NP_057067

Q9Y2X8

Human

MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM