Recombinant humaan UbcH6/UBE2E1-eiwit

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. uBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Recombinant human uBE2E1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 E1, E2 ubiquitin-conjugating enzyme E1, UbcH6, Ubiquitin carrier protein E1, Ubiquitin-protein ligase E1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.8 kDa (216aa) confirmed by MALDI-TOF

UBE2E1, ubiquitin-conjugating enzyme E2 E1 isoform 1, uBCH6, ATGP1690-10 µg, ATGP1690-20 µg, ATGP1690-50 µg, ATGP1690-100 µg, ATGP1690-250 µg, ATGP1690-500 µg, ATGP1690-1 mg, ATGP1690-10, ATGP1690-20, ATGP1690-50, ATGP1690-100, ATGP1690-250, ATGP1690-500, ATGP1690-1

Nuber u, et al. (1996) . J Biol Chem. 271 (5) : 2795-800. ; ; Takeuchi T, et al. (2005) . J. Biochem. 138 (6) : 711-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol

Ubiquitination

NP_003332

P51965

Human

MSDDDSR ASTSSSSSSS SNQQTEKETN TPKKKESKVS MSKNSKLLST SAKRIQKELA DITLDPPPNC SAGPKGDNIY EWRSTILGPP GSVYEGGVFF LDITFTPEYP FKPPKVTFRT RIYHCNINSQ GVICLDILKD NWSPALTISK VLLSICSLLT DCNPADPLVG SIATQYMTNR AEHDRMARQW TKRYAT