Recombinant humaan UBCH9/UBE2E3-eiwit

UBE2E3 also known as Ubiquitin-conjugating enzyme E2 E3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. The ubiquitination process covalently attaches ubiquitin, a short protein of 76 amino acids, to a lysine residue on the target protein. Once a protein has been tagged with one ubiquitin molecule, additional rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein that allows passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell. Recombinant human UBE2E3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 E3, E2 ubiquitin-conjugating enzyme E3, UbcH9, Ubiquitin carrier protein E3, Ubiquitin-conjugating enzyme E2-23 kDa, Ubiquitin-protein ligase E3, UBCE4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

25.3 kDa (230aa) confirmed by MALDI-TOF

UBE2E3, Ubiquitin-conjugating enzyme E2 E3, UBCH9; UbcM2, ATGP3132-10 µg, ATGP3132-20 µg, ATGP3132-50 µg, ATGP3132-100 µg, ATGP3132-250 µg, ATGP3132-500 µg, ATGP3132-1 mg, ATGP3132-10, ATGP3132-20, ATGP3132-50, ATGP3132-100, ATGP3132-250, ATGP3132-500, ATGP3132-1

Nandi, D., et al. (2006) Journal of biosciences 31 (1) : 137-55. ; ; Risseeuw, EP., et al. (2003) cell and molecular biology 34 (6) : 753-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_006348

Q969T4

Human

MGSSHHHHHH SSGLVPRGSH MGSMSSDRQR SDDESPSTSS GSSDADQRDP AAPEPEEQEE RKPSATQQKK NTKLSSKTTA KLSTSAKRIQ KELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS SDYPFKPPKV TFRTRIYHCN INSQGVICLD ILKDNWSPAL TISKVLLSIC SLLTDCNPAD PLVGSIATQY LTNRAEHDRI ARQWTKRYAT