Recombinant humaan UBD/FAT10-eiwit

UBD, also known as ubiquitin D, is an ubiquitin-like modifier (UBL) of the ubiquitin protein family. UBD is an ubiquitin-like protein that can form covalent conjugates, thereby targeting proteins for degradation by the 26S proteasome. UBD has putative roles in regulation of cell cycle, innate immunity, and apoptosis. UBD is a TNF-alpha-inducible ubiquitin-like protein with a putative role in immune response. Recombinant human UBD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ubiquitin D, Diubiquitin, FAT10, GABBR1, uBD-3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

20.9 kDa (188aa) confirmed by MALDI-TOF

Ubiquitin D, UBD-3, UBD3, UBD 3, UBD, GABBR1, GABBR 1, FAT10, FAT 10, Diubiquitin, ATGP1208-10 µg, ATGP1208-20 µg, ATGP1208-50 µg, ATGP1208-100 µg, ATGP1208-250 µg, ATGP1208-500 µg, ATGP1208-1 mg, ATGP1208-010, ATGP1208-020, ATGP1208-050, ATGP1208-100, ATGP1208-250, ATGP1208-500, ATGP1208-01M

Hipp M.S., et al. (2005) Mol. Cell. Biol. 25:3483-3491; ; Ross M.J., et al. (2006) J. Am. Soc. Nephrol. 17:996-1004

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Ubiquitination

NP_006389.2

O15205

Human

MAPNASC LCVHVRSEEW DLMTFDANPY DSVKKIKEHV RSKTKVPVQD QVLLLGSKIL KPRRSLSSYG IDKEKTIHLT LKVVKPSDEE LPLFLVESGD EAKRHLLQVR RSSSVAQVKA MIETKTGIIP ETQIVTCNGK RLEDGKMMAD YGIRKGNLLF LACYCIGG