Recombinant humaan UBE2G1-eiwit

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. uE2G1 is a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. Recombinant human uBE2G1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 G1, E2 ubiquitin-conjugating enzyme G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1, UBE2G

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

21.9 kDa (193aa) confirmed by MALDI-TOF

UBE2G1, ubiquitin-conjugating enzyme E2G 1, ubiquitin-conjugating enzyme E2G 1, E217K, uBC7, uBE2G, ATGP2890-10 µg, ATGP2890-20 µg, ATGP2890-50 µg, ATGP2890-100 µg, ATGP2890-250 µg, ATGP2890-500 µg, ATGP2890-1 mg, ATGP2890-10, ATGP2890-20, ATGP2890-50, ATGP2890-100, ATGP2890-250, ATGP2890-500, ATGP2890-1

Shibata, E., et al. (2011) Mol. Cell. Biol. 31 (15), 3136-3145; ; Hassink, G., et al. (2005) Biochem. J. 388 (PT 2), 647-655;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing, 30% glycerol, 1mM DTT

Ubiquitination

NP_003333

P62253

Human

MGSSHHHHHH SSGLVPRGSH MGSMTELQSA LLLRRQLAEL NKNPVEGFSA GLIDDNDLYR WEVLIIGPPD TLYEGGVFKA HLTFPKDYPL RPPKMKFITE IWHPNVDKNG DVCISILHEP GEDKYGYEKP EERWLPIHTV ETIMISVISM LADPNGDSPA NVDAAKEWRE DRNGEFKRKV ARCVRKSQET AFE