Recombinant humaan UBE2T-eiwit

UBE2T, also known as PIG50 or HSPC150, is a member of the E2 ubiquitin-conjugating enzyme family Involved in the protein degradation pathway. This protein catalyzes the ATP-dependent attachment of ubiquitin (ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, uBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway. Recombinant uBE2T protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Biquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, HSPC150, PIG50, FANCT

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

23.6 kDa (205aa) confirmed by MALDI-TOF

Biquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, FANCT, HSPC150, HSPC150 protein similar to ubiquitin conjugating enzyme, HSPC150 protein similar to ubiquitin-conjugating enzyme, PIG50, uBE2T, Ubiquitin carrier protein T, ubiquitin conjugating enzyme, ubiquitin conjugating enzyme E2T, ubiquitin protein ligase T, ubiquitin-conjugating enzyme E2T, ubiquitin-conjugating enzyme E2T Cell proliferation inducing gene 50 protein, Ubiquitin-protein ligase T, ATGP0529-10 µg, ATGP0529-20 µg, ATGP0529-50 µg, ATGP0529-100 µg, ATGP0529-250 µg, ATGP0529-500 µg, ATGP0529-1 mg, ATGP0529-010, ATGP0529-020, ATGP0529-050, ATGP0529-100, ATGP0529-250, ATGP0529-500, ATGP0529-01M

Hao J., et al. (2008) Tumour Biol. 29 (3) :195-203. ; ; Machida YJ., et al. (2006) Mol Cell. 23 (4) :589-96. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Ubiquitination

NP_054895

Q9NPD8

Human

MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDVLEH HHHHH