Recombinant humaan UBE2Z-eiwit

UBE2z belongs to the ubiquitin-conjugating enzyme family. This protein catalyzes the covalent attachment of ubiquitin to other proteins. It is a specific substrate for UBA6, not charged with ubiquitin by UBE1 and may be involved in apoptosis regulation. Recombinant human UBE2Z protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-conjugating enzyme E2 Z, E2 ubiquitin-conjugating enzyme Z, Uba6-specific E2 conjugating enzyme 1, Use1, Ubiquitin carrier protein Z, Ubiquitin-protein ligase Z, HOYS7

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.5 kDa (269aa) confirmed by MALDI-TOF

Use1, UBE2Z, ubiquitin-conjugating enzyme E2Z, HOYS7, uSE1, ATGP2726-10 µg, ATGP2726-20 µg, ATGP2726-50 µg, ATGP2726-100 µg, ATGP2726-250 µg, ATGP2726-500 µg, ATGP2726-1 mg, ATGP2726-10, ATGP2726-20, ATGP2726-50, ATGP2726-100, ATGP2726-250, ATGP2726-500, ATGP2726-1

Gu X, Zhao F., et al. (2007) Mol Biol Rep. 34 (3) :183-8

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Ubiquitination

AAH15890

Q9H832

Human

MSIYKEP PPGMFVVPDT VDMTKIHALI TGPFDTPYEG GFFLFVFRCP PDYPIHPPRV KLMTTGNNTV RFNPNFYRNG KVCLSILGTW TGPAWSPAQS ISSVLISIQS LMTENPYHNE PGFEQERHPG DSKNYNECIR HETIRVAVCD MMEGKCPCPE PLRGVMEKSF LEYYDFYEVA CKDRLHLQGQ TMQDPFGEKR GHFDYQSLLM RLGLIRQKVL ERLHNENAEM DSDSSSSGTE TDLHGSLRV