Recombinant humaan UBL3-eiwit

UBL3, also known as PNSC1, is a 117 amino acid membrane protein belonging to the ubiquitin-like family. This protein contains two potential N-glycosylation sites, a potential protein kinase C phosphorylation site and a potential C-terminal prenylation site. ubiquitin-like proteins are not directly involved in protein degradation, but appear to have many mechanistic similarities with the ubiquitin pathway. Recombinant human uBL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin-like protein 3, DKFZp434K151, FLJ32018, HCG-1, PNSC1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 95% by SDS-PAGE

15 kDa (134aa) confirmed by MALDI-TOF

UBL3, ubiquitin-like protein 3, DKFZp434K151, FLJ32018, HCG-1, PNSC1, ATGP1081-10 µg, ATGP1081-20 µg, ATGP1081-50 µg, ATGP1081-100 µg, ATGP1081-250 µg, ATGP1081-500 µg, ATGP1081-1 mg, ATGP1081-10, ATGP1081-20, ATGP1081-50, ATGP1081-100, ATGP1081-250, ATGP1081-500, ATGP1081-1

Downes BP., et al. (2006) J Biol Chem. 281 (37) :27145-57.; ; Leung A., et al. (2008) Ernst Schering Found Symp Proc. (1) :75-97.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl.

Ubiquitination

NP_009037

O95164

Human

MSSNVPADMI NLRLILVSGK TKEFLFSPND SASDIAKHVY DNWPMDWEEE QVSSPNILRL IYQGRFLHGN VTLGALKLPF GKTTVMHLVA RETLPEPNSQ GQRNREKTGE SNCC