Recombinant humaan UBL4A-eiwit

UBL4A, also known as GDX, contains 1 ubiquitin-like domain. Post-translational modification by ubiquitin and ubiquitin-related proteins plays critical roles in protein degradation and in regulation of essential cellular processes. In mammals, transcription grinds to a halt during late spermiogenesis due to compaction of the spermatid genome, which creates a special need for robust post-transcriptional regulation. uBL4A plays any role in targeting cellular proteins for degradation. Recombinant human uBL4A protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin like 4A, Ubiquitin-like 4, UBL4, Ubiquitin-like protein GDX, DXS254E, GET5, MDY2, TMA24

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

18.8 kDa (165aa) confirmed by MALDI-TOF

UBL4A, ubiquitin-like protein 4A, G6PD, GDX, GET5, MDY2, TMA24, uBL4, ATGP1418-10 µg, ATGP1418-20 µg, ATGP1418-50 µg, ATGP1418-100 µg, ATGP1418-250 µg, ATGP1418-500 µg, ATGP1418-1 mg, ATGP1418-10, ATGP1418-20, ATGP1418-50, ATGP1418-100, ATGP1418-250, ATGP1418-500, ATGP1418-1

Yang F, et al. (2007) Gene Expr Patterns. 7 (1-2) :131-6. ; ; Mariappan M., et al. (2010) Nature 466:1120-1124

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Ubiquitination

NP_055050

P11441

Human

MQLTVKALQG RECSLQVPED ELVSTLKQLV SEKLNVPVRQ QRLLFKGKAL ADGKRLSDYS IGPNSKLNLV VKPLEKVLLE EGEAQRLADS PPPQVWQLIS KVLARHFSAA DASRVLEQLQ RDYERSLSRL TLDDIERLAS RFLHPEVTET MEKGFSKLEH HHHHH