Recombinant humaan UBTD2-eiwit

UBTD2, also known as DCuBP (dendritic cellderived ubiquitin-like protein), is a ubiquitin (ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. ubiquitin is the most well understood post-translation modifier, there is a growing family of ubiquitin-like proteins (uBLs) that modify cellular targets in a pathway. ubL proteins are involved in a variety of cellular processes, including DNA repair, protein sorting, apoptosis, protein degradation, cell division and autophagy. Recombinant human uBTD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin domain containing 2, DCuBP, SB72

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

28.6 kDa (257aa) confirmed by MALDI-TOF

UBTD2, ubiquitin domain containing 2, DCuBP, SB72, ATGP1697-10 µg, ATGP1697-20 µg, ATGP1697-50 µg, ATGP1697-100 µg, ATGP1697-250 µg, ATGP1697-500 µg, ATGP1697-1 mg, ATGP1697-10, ATGP1697-20, ATGP1697-50, ATGP1697-100, ATGP1697-250, ATGP1697-500, ATGP1697-1

Song AX, et al. (2010) Protein Sci. 19 (5) :1104-9.; ; Gao, Y.G., et al. (2005) Protein Sci. 14: 2044-2050.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Ubiquitination

NP_689490

Q8WUN7

Human

MGGCVGA QHDSSGSLNE NSEGTGVALG RNQPLKKEKP KWKSDYPMTD GQLRSKRDEF WDTAPAFEGR KEIWDALKAA AHAFESNDHE LAQAIIDGAN ITLPHGALTE CYDELGNRYQ LPVYCLAPPI NMIEEKSDIE TLDIPEPPPN SGYECQLRLR LSTGKDLKLV VRSTDTVFHM KRRLHAAEGV EPGSQRWFFS GRPLTDKMKF EELKIPKDYV VQVIVSQPVQ NPTPVEN