Recombinant humaan UCHL3-eiwit

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as uCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. uCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. uCHL3 protein is 54% identical to that of uCHL1. Recombinant human uCHL3 protprot, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

UCH-L3, ubiquitin thioesterase L3, ubiquitin carboxyl-terminal hydrolase isozyme L3, ubiquitin carboxyl-terminal esterase L3

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 1,500pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

28.3 kDa (250aa) confirmed by MALDI-TOF

HUMAN UCHL3, Ubiquitin carboxyl terminal esterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCHL3, UCH-L3, UCHL3 HUMAN, UCHL3_HUMAN, UCHL3HUMAN, ATGP0872-10 µg, ATGP0872-20 µg, ATGP0872-50 µg, ATGP0872-100 µg, ATGP0872-250 µg, ATGP0872-500 µg, ATGP0872-1 mg, ATGP0872-010, ATGP0872-020, ATGP0872-050, ATGP0872-100, ATGP0872-250, ATGP0872-500, ATGP0872-01M

Kwon J., et al. (2007) Exp Anim. 56:71-7.; ; Leroy E., et al. (1998) Nature. 395:451-2.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Ubiquitination

NP_005993

P15374

Human

MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA