Recombinant humaan UDP-galactose-4-epimerase/GALE-eiwit

GALE, also known as uDP-glucose 4-epimeraes, is a protein that functions as the third enzyme in the Leloir pathway of galactose metabolism. It is a homodimeric epimerase found in bacterial, plant, and mammalian cells. This enzyme promotes the reverse chemical reaction, the conversion of uDP-glucose to uDP-galactose. uDP-galactose is used to build galactose-containing proteins and fats, which play critical roles in chemical signaling, building cellular structures, transporting molecules, and producing energy. Recombinant human GALE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-galactose 4-epimerase, short chain dehydrogenase/reductase family 1E, member 1, SDR1E1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

40.4 kDa (368aa) confirmed by MALDI-TOF

GALE, uDP-glucose 4-epimeraes, SDR1E1.uDP-glucose 4-epimeraes, uDP-galactose 4-epimerase, Galactowaldenase, FLJ95174, FLJ97302, ATGP0614-10 µg, ATGP0614-20 µg, ATGP0614-50 µg, ATGP0614-100 µg, ATGP0614-250 µg, ATGP0614-500 µg, ATGP0614-1 mg, ATGP0614-10, ATGP0614-20, ATGP0614-50, ATGP0614-100, ATGP0614-250, ATGP0614-500, ATGP0614-1

Holden HM., et al. (2003) J Biol Chem. 278 (45) :43885-8. ; ; Timson DJ., et al. (2005) FEBS J. 272 (23) :6170-7. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 5mM DTT, 0.1M NaCl, and 1mM EDTA

Enzymes & Proteases

NP_000394.2

Q14376

Human

MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA