Recombinant humaan UDP-glucosedehydrogenase/UGDH-eiwit

UGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Specific activity is > 2500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 2500pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

59.5 kDa (533aa) confirmed by MALDI-TOF

UGDH, uDP-glucose dehydrogenase, GDH, uDP-GlcDH, uDPGDH, uGD, uDP-glucose dehydrogenase, uDP-glucose 6-dehydrogenase, ATGP0611-10 µg, ATGP0611-20 µg, ATGP0611-50 µg, ATGP0611-100 µg, ATGP0611-250 µg, ATGP0611-500 µg, ATGP0611-1 mg, ATGP0611-10, ATGP0611-20, ATGP0611-50, ATGP0611-100, ATGP0611-250, ATGP0611-500, ATGP0611-1

Bontemps Y., et al. (2003) J Biol Chem. 278 (24) :21566-75. ; ; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70 (2) :401-10. ;

Hagiuda D, et al. Clinicopathological and prognostic significance of nuclear UGDH localization in lung adenocarcinoma. (Biomed Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/30787260/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA

Enzymes & Proteases

NP_003350.1

O60701

Human

M FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV