Recombinant humaan Ufc1-eiwit

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. uFM1 is activated by a novel E1-like enzyme, uBA5, by forming a high-energy thioester bond. Activated uFM1 is then transferred to its cognate E2-like enzyme, uFC1, in a similar thioester linkage. Recombinant human uFC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Ubiquitin-fold modifier-conjugating enzyme 1, HSPC155

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.6 kDa (187aa) confirmed by MALDI-TOF

Ufc1, ubiquitin-fold modifier-conjugating enzyme 1, HSPC155, ATGP1063-10 µg, ATGP1063-20 µg, ATGP1063-50 µg, ATGP1063-100 µg, ATGP1063-250 µg, ATGP1063-500 µg, ATGP1063-1 mg, ATGP1063-10, ATGP1063-20, ATGP1063-50, ATGP1063-100, ATGP1063-250, ATGP1063-500, ATGP1063-1

Komatsu M. et al. (2004) EMBO J. 23 :1977-1986.; ; Tatsumi K. et al. (2010) J Biol Chem. 285:5417-5427.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Ubiquitination

NP_057490

Q9Y3C8

Human

MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ