Recombinant humaan UFD1L-eiwit

UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. Recombinant human UFD1L protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ubiquitin fusion degradation protein 1 homolog isoform A, UFD1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.9 kDa (330aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

UFD1L, ubiquitin fusion degradation protein 1 homolog isoform A, uFD1, ATGP2241-10 µg, ATGP2241-20 µg, ATGP2241-50 µg, ATGP2241-100 µg, ATGP2241-250 µg, ATGP2241-500 µg, ATGP2241-1 mg, ATGP2241-10, ATGP2241-20, ATGP2241-50, ATGP2241-100, ATGP2241-250, ATGP2241-500, ATGP2241-1

Hass A L., et al. (1997) FASEB. 11:1257-1268; ; Novelli G., et al. (1998) Biochim Biophys Acta. 1396:158-162.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT

Ubiquitination

NP_005650

Q92890

Human

MFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP